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1x reagent diluent concentrate 2 (R&D Systems)

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R&D Systems 1x reagent diluent concentrate 2
1x Reagent Diluent Concentrate 2, supplied by R&D Systems, used in various techniques. Bioz Stars score: 95/100, based on 155 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/reagent+diluent+concentrate+2/Reagent+Diluent+Concentrate+2/pm42021300-96-13-18
Average 95 stars, based on 155 article reviews

1x reagent diluent concentrate 2 - by Bioz Stars, 2026-10

95/100 stars

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Saline:

Article Title: On-site analysis of cortisol in saliva based on microchannel lateral flow assay (mLFA) on polymer lab-on-a-chip (LOC).
Article Snippet: Rabbit anti-mouse IgG (RIgG), ab6709 (Abcam, USA) was used as a capture antibody in positive control spiral reaction channels, having a specific affinity in binding directly to the DAb-AF488. .. Reagent Diluent Concentrate 2 containing 1% bovine serum albumin (BSA) in phosphate-buffered saline (PBS) (10 mg/mL BSA in PBS), DY995 (R&D systems, USA) was used as a blocking buffer. .. Phosphate-buffered saline (1X PBS), DY006 (R&D systems, USA) was used as washing buffer in the Opti-96TM microplate.

Article Title: On-site analysis of cortisol in saliva based on microchannel lateral flow assay (mLFA) on polymer lab-on-a-chip (LOC)
Article Snippet: Rabbit anti-mouse IgG (RIgG), ab6709 (Abcam, USA) was used as a capture antibody in positive control spiral reaction channels, having a specific affinity in binding directly to the DAb-AF488. .. Reagent Diluent Concentrate 2 containing 1% bovine serum albumin (BSA) in phosphate-buffered saline (PBS) (10 mg/mL BSA in PBS), DY995 (R&D systems, USA) was used as a blocking buffer. .. Phosphate-buffered saline (1X PBS), DY006 (R&D systems, USA) was used as washing buffer in the Opti-96TM microplate.

Blocking Assay:

Enzyme-linked Immunosorbent Assay:

Article Title: VISTA is an activating receptor in human monocytes
Article Snippet: Streptavidin-HRP (016-030-084) was purchased from Jackson ImmunoResearch Laboratories. .. The ELISA Substrate Reagent Pack (DY999), ELISA plate-coating buffer (DY006), reagent diluent concentrate 2 (DY995), and Stop Solution (DY994) were purchased from R&D Systems. ..

Article Title: Improving a Plasma Biomarker Panel for Early Detection of Pancreatic Ductal Adenocarcinoma with Aminopeptidase N (ANPEP) and Polymeric immunoglobin receptor (PIGR)
Article Snippet: ANPEP plates were prepared following manufacturer instructions using DuoSet ELISA Ancillary Reagent Kit 2 (R&D Systems Cat. #DY008B) by adding 100 uL of diluted capture antibody to each well and incubating at room temperature overnight then blocked with 300 uL of Protein-Free T20 blocking buffer (Thermo Scientific TM Cat# 37573), before continuing the protocol provided by manufacturer. .. ANPEP samples were diluted in 1% BSA in PBS using R&D Systems ® Reagent Diluent Concentrate 2 (Catalog # DY995) from DuoSet ELISA Ancillary Reagent Kit 2. .. Plates for all assays were read at 450 nm using Molecular Devices SpectraMax 340PC384 Microplate Reader (RRID:SCR_020303) and SoftMax Pro (RRID:SCR_014240) version 5.3.